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KMID : 0364820150510030221
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Korean Journal of Microbiology
2015 Volume.51 No. 3 p.221 ~ p.230
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Microbial community structures in the ground bed cultivation of Lentinula edodes using oak sawdust
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Shin Ji-Hye
Yun Seo-Yeon
Nam Ji-Hyun
Koo Chang-Duck
Lee Dong-Hun
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Abstract
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Ground bed cultivation method for shiitake mushroom (Phogo; Lentinula edodes) has been advanced to use the oak-sawdust composted by microorganisms. This method has some advantages in saving the labors and improving productivity. The aim of this study is to analyze microbial community in oak sawdust and to provide the information for efficient cultivation of shiitake mushroom. In process of composting, the proportions of thermophiles increased from 10% to 80%. This results show the microbial community succession from mesophilic bacteria to thermophilic bacteria. T-RFLP analysis and nucleotide sequencing of 16S rRNA gene demonstrated that the change of dominant bacteria coupled with shifts in each stages. The family of Enterobacteriaceae as a mesophilic bacteria were dominant (100%) in oak sawdust sample, but Amycolatopsis (49.0%) and Saccharopolyspora (26.5%) as a thermophilic Actinomycetes were dominant in the last day of composting. In hyphal growth stage, Leuconostoc (psychrophilic bacteria) was dominant (75.0%). The
composting process of sawdust is very important for growth of mushroom mycelium. Therefore, there is need for the further study for the Amycolatopsis as thermophilic Actinomycetes.
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KEYWORD
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Amycolatopsis, Lentinula edodes, composting, oak sawdust, shiitake mushroom
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